人溶菌酶密码子优化及其在牛乳腺细胞中高效表达

田媛,王力,龙凤,昝林森,成功

Codon Optimization of Human Lysozyme and High-Efficiency Expression in Bovine Mammary Cells

TIAN Yuan,WANG Li,LONG Feng,ZAN LinSen,CHENG Gong

表1 7种牛乳蛋白基因GenBank登录号及序列信息

Table 1 GenBank accession number and sequence information of cattle seven milk protein genes

基因名称 Gene name GenBank号 GenBank No. 序列长度 Length of CDS alpha-s1 casein (CSN1S1) M33123 645 bp alpha-s2 casein (CSN1S2) BC114773 669 bp beta casein (CSN2) NM_181008 675 bp kappa casein (CSN3) BC102120 573 bp beta-lactoglogbulin (LGB) NM_173929 537 bp alpha-lactalbumin (LALBA) M18780 429 bp lactotransferrin (LTF) NM_180998 2127 bp