莲藕多酚氧化酶互作蛋白的筛选及验证
原新博1(
),程婷婷1,惠小涵1,陈章玉1,王瑞红2,柯卫东3,郭宏波1()
),程婷婷1,惠小涵1,陈章玉1,王瑞红2,柯卫东3,郭宏波1()
Screening of Polyphenol Oxidase Interaction Proteins from Nelumbo nucifera and Their Verification
YUAN XinBo1(),CHENG TingTing1,XI XiaoHan1,CHEN ZhangYu1,WANG RuiHong2,KE WeiDong3,GUO HongBo1()
),CHENG TingTing1,XI XiaoHan1,CHEN ZhangYu1,WANG RuiHong2,KE WeiDong3,GUO HongBo1()
图1. 基因的克隆及互作蛋白筛选
A：以莲藕cDNA为模板扩增7个抗氧化基因的电泳图。M：DL2000分子标记;1：APX;2：CuZnSOD;3：PER1;4：POD3L;5：POD43L;6：PODP7L;7：NnCAT1 B：酵母双杂交筛选与莲藕JDPPO1互作的蛋白
Fig. 1. Gene cloning and screening of interacting proteins
A: Amplification of seven genes using Nelumbo nucifera cDNA as template. M：DL2000 marker; 1: APX; 2: CuZnSOD; 3: PER1; 4: POD3L; 5: POD43L; 6: PODP7L; 7: NnCAT1; B: Screening of JDPPO1 interacting proteins by yeast two-hybrid
