柑橘脉突病毒基因组全长cDNA克隆及其侵染性鉴定
许建建(
),王艳娇(),段玉,马志敏,宾羽,周常勇,宋震()
),王艳娇(),段玉,马志敏,宾羽,周常勇,宋震()
Construction of Genome-Length cDNA of Citrus Vein Enation Virus and Identification of Its Infectivity
XU JianJian(),WANG YanJiao(),DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen()
),WANG YanJiao(),DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen()
图5. CVEV全长cDNA克隆接种尤力克柠檬的RT-PCR检测
M：2000 bp分子量marker 2000 bp molecular marker;1—5：pTX1空载接种对照 pTX1 no-load inoculation control;6—19：CVEV1901接种样品 CVEV1901 inoculated samples;20—34：CVEV1902接种样品 CVEV1902 inoculated samples;35：阴性对照Negative control;36：ddH2O;37：阳性对照CVEV XZG Positive control CVEV XZG
Fig. 5. RT-PCR detection of C. limon inoculated with full-length cDNA of CVEV
