柑橘脉突病毒基因组全长cDNA克隆及其侵染性鉴定
许建建(
),王艳娇(),段玉,马志敏,宾羽,周常勇,宋震()
),王艳娇(),段玉,马志敏,宾羽,周常勇,宋震()
Construction of Genome-Length cDNA of Citrus Vein Enation Virus and Identification of Its Infectivity
XU JianJian(),WANG YanJiao(),DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen()
),WANG YanJiao(),DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen()
图1. CVEV 5′末端RACE及克隆
M：DNA分子标准 DNA marker;1、2：5′末端RACE 5′ RACE ;7—9：5′末端cDNA克隆PCR检测 Detection of cDNA clones of 5′-end sequence using PCR;3—5：阴性对照 Negative control;6：ddH2O
Fig. 1. 5′ rapid amplification of cDNA ends (RACE) and its cloning of CVEV
