柑橘脉突病毒基因组全长cDNA克隆及其侵染性鉴定

许建建,王艳娇,段玉,马志敏,宾羽,周常勇,宋震

Construction of Genome-Length cDNA of Citrus Vein Enation Virus and Identification of Its Infectivity

XU JianJian,WANG YanJiao,DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen

表1 引物序列

Table 1 Primer sequences

引物Primer 序列Sequence (5′→3′) EV532R CGAGCGGCACAAATTCTAAAGTACCAC EV414R GACCCCATAGCAAAGCAAGAGCACAT UPM long CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT NUP AAGCAGTGGTATCAACGCAGAGT SMARTer II A Oligonucleotide AAGCAGTGGTATCAACGCAGAGTACXXXXX EV25-F TTTCATTTGGAGAGGAATATAAGCTATAAAAGAAAAGTGCG EV5983-R ATGCCATGCCGACCCACAAAAGTATGCAAAGAATATG VE16-F AATTACGGCGTATCTATGGTGAGTCG VE17-R AATGAGATAGCCCGGTTGTCCAG