甘肃、青海地区小麦条锈菌监测及群体遗传多样性分析

黄苗苗,陈万权,曹世勤,孙振宇,贾秋珍,高利,刘博,刘太国

Surveillance and Genetic Diversity Analysis of Puccinia striiformis f. sp. tritici in Gansu and Qinghai Provinces

HUANG MiaoMiao,CHEN WanQuan,CAO ShiQin,SUN ZhenYu,JIA QiuZhen,GAO Li,LIU Bo,LIU TaiGuo

表2 本试验使用的15对SSR小麦条锈菌引物信息

Table 2 Information of the 15 SSR primers for Pst used in this study

引物名称 Primer name	重复单元 Repeat motif		引物序列 Sequence (5′-3′)	染料 Dye	退火温度 Tm (℃)	参考文献 Reference
RJ03	(TGG)₈	F:	GCAGCACTGGCAGGTGG	FAM	61	文献[15] Reference [15]
		R:	GATGAATCAGGATGGCTCC			文献[15] Reference [15]
RJ04	(TGG)₈	F:	GTGGGTTGGGCTGGAGTC	HEX	58	文献[15] Reference [15]
		R:	GCTAATCCATTCCACGCACC			文献[15] Reference [15]
RJ12	(AC)₇	F:	ATC ATT CCG ATT TCT TTC TCA CC	FAM	56	文献[15] Reference [15]
		R:	TCA CAC TGA TCC CAA TAG ATC AG		56	文献[15] Reference [15]
RJ15	(TG)₇	F:	ATC GAG CAC GTC CAA ATC G	HEX	56	文献[15] Reference [15]
		R:	CAC TGG ACA GAC GAC GGT TG		56	文献[15] Reference [15]
RJ18	(TGT)₅	F:	CTG CCC ATG CTC TTC GTC	ROX	59	文献[15] Reference [15]
		R:	GAT GAA GTG GGT GCT GCT G		59	文献[15] Reference [15]
RJ20	(CAG)₄	F:	AGA AGA TCG ACG CAC CCG	ROX	56	文献[15] Reference [15]
		R:	CCT CCG ATT GGC TTA GGC		56	文献[15] Reference [15]
RJ21	(GTT)₆	F:	TTC CTG GAT TGA ATT CGT CG	FAM	55	文献[15] Reference [15]
		R:	CAG TTC TCA CTC GGA CCC AG		55	文献[15] Reference [15]
RJ24	(GTT)₅₊₉	F:	TTG CTG AGT AGT TTG CGG TGA G	HEX	58	文献[15] Reference [15]
		R:	CTC AAG CCC ATC CTC CAA CC			文献[15] Reference [15]
RJ27	(TC)₁₀	F:	CGTCCCGACTAATCTGGTCC	ROX	52	文献[15] Reference [15]
		R:	ATGAGTTAGTTTAGATCAGGTCGAC			文献[15] Reference [15]
CPS08	(CAG)₁₄	F:	GAT AAG AAA CAA GGG ACA GC	FAX	56	文献[2] Reference [2]
		R:	CAG TGA ACC CAA TTA CTC AG			文献[2] Reference [2]
CPS09	(GTT)₉	F:	CGG GAG AAG ACC TGA GC	ROX	55	文献[2] Reference [2]
		R:	AGA AAA CGG AAT GTA ATG TG			文献[2] Reference [2]
CPS10	(TAG)₈	F:	TCT ACT GGG CAG ACT GGT C	HEX	56	文献[2] Reference [2]
		R:	CGG TTT GTT TTG TCG TTT C			文献[2] Reference [2]
CPS15	(ATG)₅	F:	GAT GGG GAA AAG TAA GAA GT	FAM	56	文献[2]
		R:	GGT GGG GGA TGT AAG TAT GTA			Reference [2]
CPS34	(TTTGG)₄	F:	GTT GGC TAC GAG TGG TCA TC	HEX	56	文献[2]
		R:	TAA CAC TAC ACA AAA GGG GTC			Reference [2]
CPS36	(CTCTAG)₃	F:	TCC AGG CAG TAA ATC AGA CGC	ROX	58	文献[2]
		R:	ATC AGC AGG TGT AGC CCC ATC			Reference [2]