紫花苜蓿MsDWF4的表达特性及耐盐性效应

崔苗苗,马琳,张锦锦,王筱,庞永珍,王学敏

Gene Expression and Salt-Tolerance Analysis of MsDWF4 Gene from Alfalfa

CUI MiaoMiao,MA Lin,ZHANG JinJin,WANG Xiao,PANG YongZhen,WANG XueMin

表2 本试验所用引物

Table 2 Primers used in this research

引物名称 Primer name 引物序列 Primer sequence (5′-3′) ORF-F CACCCTATTGGCTTCACA ORF-R GAAAGTGGTCCTATTGACA qDF AACAAAACATGCCAAACCCAA qDR CCATTTCCCAAGTATTCCACCA Msactin-F CAAAAGATGGCAGATGCTGAGGAT Msactin-R CATGACACCAGTATGACGAGGTCG pMsDWF4-F ACGGGGGACGAGCTCGGTACCATGTCTGACTCAGATGTAACTT pMsDWF4-R CTTGCTCACCATGTCGACTCTAGATATTATAGAGTGGGCTTGGACTCTAATTTGTAGG MDF CCACTGACGTAAGGGATGACG MDR TGTCGAAACCGATGATACGAACGAA