农杆菌介导大麦无筛选标记转基因植株的获得
龚强1,2(
),王轲2(),叶兴国2,杜丽璞2,徐延浩1()
),王轲2(),叶兴国2,杜丽璞2,徐延浩1()
Generation of Marker-Free Transgenic Barley Plants by Agrobacterium-Mediated Transformation
GONG Qiang1,2(),WANG Ke2(),YE XingGuo2,DU LiPu2,XU YanHao1()
),WANG Ke2(),YE XingGuo2,DU LiPu2,XU YanHao1()
图8. T1代转基因大麦植株的Southern blot鉴定
A：Bar的Southern blot鉴定;B：GUS的Southern blot鉴定。WT：受体对照;M：DL15000 marker;BL8-15和BL8-19为筛选到的无筛选标记转基因植株
Fig. 8. Southern blot detection of T1 transgenic barley plants
A: Southern blot of Bar; B: Southern blot of GUS. WT: Wild type; M: Marker; BL8-15 and BL8-19were marker-free transgenic plants
