农杆菌介导大麦无筛选标记转基因植株的获得
龚强1,2(
),王轲2(),叶兴国2,杜丽璞2,徐延浩1()
),王轲2(),叶兴国2,杜丽璞2,徐延浩1()
Generation of Marker-Free Transgenic Barley Plants by Agrobacterium-Mediated Transformation
GONG Qiang1,2(),WANG Ke2(),YE XingGuo2,DU LiPu2,XU YanHao1()
),WANG Ke2(),YE XingGuo2,DU LiPu2,XU YanHao1()
图7. T1代转基因大麦株系BL8中Bar（A）和GUS（B）的PCR检测
1—22：T1转基因株系BL8;23：Vlamingh;P：pWMB123质粒;M：DL2000 marker;15和19为筛选到的无筛选标记转基因植株
Fig. 7. PCR detection of Bar (A) and GUS (B) genes in T1 transgenic barley plants
1-22: BL-8 of T1 transgenic barley plants; 23: Vlamingh; P: pWMB123 plasmid; M: DL2000 marker; 15 and 19 were marker-free transgenic plants
