农杆菌介导大麦无筛选标记转基因植株的获得
龚强1,2(
),王轲2(),叶兴国2,杜丽璞2,徐延浩1()
),王轲2(),叶兴国2,杜丽璞2,徐延浩1()
Generation of Marker-Free Transgenic Barley Plants by Agrobacterium-Mediated Transformation
GONG Qiang1,2(),WANG Ke2(),YE XingGuo2,DU LiPu2,XU YanHao1()
),WANG Ke2(),YE XingGuo2,DU LiPu2,XU YanHao1()
图6. T0代转基因大麦植株鉴定
A：Bar蛋白抗体试纸条检测;B：GUS组织化学染色;C：GUS的PCR检测。1—14是大麦T0代转基因植株;WT：Vlamingh;P：pWMB123质粒;M：DL5000 marker
Fig. 6. Identification of T0 transgenic barley plants
A: Detection of Bar protein by Quick Stix strips; B: Detection of GUS gene by histochemical staining; C: Detection of GUS gene by PCR. 1-14: T0 transgenic barley plants; WT: Vlamingh; P: pWMB123 plasmid; M: DL5000 marker
