农杆菌介导大麦无筛选标记转基因植株的获得

龚强,王轲,叶兴国,杜丽璞,徐延浩

Generation of Marker-Free Transgenic Barley Plants by Agrobacterium-Mediated Transformation

GONG Qiang,WANG Ke,YE XingGuo,DU LiPu,XU YanHao

表3 T1代株系中Bar和GUS的分离情况

Table 3 Genotyping detection for the Bar and GUS genes in the selected T1 lines by PCR

株系 Lines Bar+GUS+ Bar+GUS— Bar—GUS+ Bar—GUS— BL1 19 1 0 0 BL6 23 0 0 0 BL-7 24 2 0 0 BL-8 27 0 2 0