蜜蜂球囊菌菌丝和孢子中微小RNA及其靶mRNA的比较分析

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Comparative Analysis of MicroRNAs and Corresponding Target mRNAs in Ascosphaera apis Mycelium and Spore

CHEN HuaZhi,ZHU ZhiWei,JIANG HaiBin,WANG Jie,FAN YuanChan,FAN XiaoXue,WAN JieQi,LU JiaXuan,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui

表1 本研究使用的引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列Primer sequence (5′-3′) Loop-miR5658-x CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCATCATC Loop-miR-10285-y CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACAATTGG Loop-miR-3245-y CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCCCGGAC Loop-miR4404-x CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGCCAGTCT Loop-miR-9-z CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCATACAG miR5658-x-F ACACTCCAGCTGGGCGATGATGAT miR-10285-y-F ACACTCCAGCTGGGTCATTTGTGGT miR-3245-y-F ACACTCCAGCTGGGCTTGGGAGAG miR4404-x-F ACACTCCAGCTGGGAATACGTAGA miR-9-z-F ACACTCCAGCTGGGTCTTTGGTTATCTAG Universal-R CTCAACTGGTGTCGTGGA actin-F CAGGAAAGGCTATGTTCGC actin-R ATTACCGAGGAGCAAGACG