紫花苜蓿MsWRKY42的分离、鉴定及其对非生物胁迫的响应

刘佼佼,王学敏,马琳,崔苗苗,曹晓宇,赵威

Isolation, Identification, and Response to Abiotic Stress of MsWRKY42 Gene from Medicago sativa L.

LIU JiaoJiao,WANG XueMin,MA Lin,CUI MiaoMiao,CAO XiaoYu,ZHAO Wei

表1 PlantCARE 启动子预测结果

Table 1 Promoter prediction by PlantCARE

顺式作用元件 Cis-elements 序列 Sequence (5′-3′) 功能 Function 数量 No. CGTCA-motif CGTCA 参与MeJA反应的顺式作用调节元件 Cis-acting regulatory element involved in the MeJA-responsiveness 2 GARE-motif TCTGTTG 赤霉素反应元件 Gibberellin-responsive element 1 I-box GTATAAGGCC 部分光响应元件 Part of a light responsive element 1 Box 4 ATTAAT 参与光响应的保守 DNA 模块的部分元件 Part of a conserved DNA module involved in light responsiveness 5 HD-Zip 3 GTAAT(G/C)ATTAC 蛋白质结合位点 Protein binding site 1 circadian CAAAGATATC 参与昼夜节律顺行调控元件 Cis-acting regulatory element involved in circadian control 1 TCCC-motif TCTCCCT 部分光响应元件 Part of a light responsive element 1 TGACG-motif TGACG 参与MeJA反应的顺式作用调节元件 Cis-acting regulatory element involved in the MeJA-responsiveness 2 3-AF3 binding site CACTATCTAAC 保守DNA模块阵列的一部分（CMA3） Part of a conserved DNA module array (CMA3) 1 TATC-box TATCCCA 参与赤霉素反应的顺式作用元件 Cis-acting element involved in gibberellin-responsiveness 1 Sp1 GGGCGG 光响应元件 Light responsive element 1 TC-rich repeats ATTCTCTAAC 参与防御和胁迫反应的顺式作用元件 Cis-acting element involved in defense and stress responsiveness 1 GCN4_motif TGAGTCA 参与胚乳表达的顺式调控元件 Cis-regulatory element involved in endosperm expression 1 CAAT-box CAAAT 启动子和增强子区域中常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions 16 G-Box CACGTG 参与光响应顺式作用元件 Cis-acting regulatory element involved in light responsiveness 5 GATA-motif GATAGGG 部分光响应元件 Part of a light responsive element 1 O2-site GATGA(C/T)(A/G)TG(A/G) 参与玉米醇溶蛋白代谢调节的顺式作用调节元件 Cis-acting regulatory element involved in zein metabolism regulation 1 GT1-motif GGTTAAT 光响应元件 Light responsive element 2 TATA-box TATA 在转录起始的-30 附近的核心启动子元件 Core promoter element around -30 of transcription start 41 ARE AAACCA 厌氧诱导必需元件 Cis-acting regulatory element essential for the anaerobic induction 2 GC-motif CCCCCG 参与缺氧特异性诱导的类似增强子元件 Enhancer-like element involved in anoxic specific inducibility 1 TCA-element CCATCTTTTT 参与水杨酸响应的顺式作用元件 Cis-acting element involved in salicylic acid responsiveness 1 TCT-motif TCTTAC 部分光响应元件 Part of a light responsive element 2 ATCT-motif AATCTAATCC 部分参与光响应的保守DNA区域 Part of a conserved DNA module involved in light responsiveness 2 ABRE CACGTG 参与脱落酸反应的顺式作用元件 Cis-acting element involved in the abscisic acid responsiveness 5