芝麻肌醇半乳糖苷合成酶基因SiGolS6的克隆及功能分析
刘爱丽(
),魏梦园,黎冬华,周瑢,张秀荣,游均()
),魏梦园,黎冬华,周瑢,张秀荣,游均()
Cloning and Function Analysis of Sesame Galactinol Synthase Gene SiGolS6 in Arabidopsis
LIU AiLi(),WEI MengYuan,LI DongHua,ZHOU Rong,ZHANG XiuRong,YOU Jun()
),WEI MengYuan,LI DongHua,ZHOU Rong,ZHANG XiuRong,YOU Jun()
图2. 转基因拟南芥的获得和鉴定
A、B：含潮霉素的MS筛选培养基上生长的T2转基因（A）和野生型（B）植株;C：T2阳性转基因植株鉴定,1—6：转基因株系;WT：野生型对照;D：转基因株系中SiGolS6的表达量检测。*表示在P<0.05水平上差异显著;**表示在P<0.01水平上差异显著。下同
Fig. 2. Acquisition and identification of transgenic Arabidopsis
A, B: Transgenic (A) and wild type (B) plants growing on selective medium containing hygromycin; C: Identification of positive T2 generation transgenic Arabidopsis plants; 1-6: SiGolS6 transgenic lines; WT: Wild type; D: Transcript level of SiGolS6 in transgenic Arabidopsis plants. * indicate significant difference at P<0.05 level; **indicates significant difference at P<0.01 level. The same as below
