辣椒脉斑驳病毒的多基因联合检测与鉴定
杨宏凯1(
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
Multi-Gene-Based PCR Detection and Identification of Chilli veinal mottle virus
YANG HongKai1(),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
图5. 多基因联合检测的特异性
A：多基因联合检测;B：基于CP的PCR检测PCR; C：基于CI的PCR检测PCR。M：DNA分子量标准;1：辣椒脉斑驳病毒;2—5：其他辣椒主要病毒;6：阴性对照
Fig. 5. Specificity of the multi-gene-based PCR detection
A：Multi-gene-based PCR detection;B：assay based on CP; C：assay based on CI。Marker DNA (100 bp);1：ChiVMV;2—5：Other main viruses of chilli;6：Negative control (healthy plant)
