辣椒脉斑驳病毒的多基因联合检测与鉴定
杨宏凯1(
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
Multi-Gene-Based PCR Detection and Identification of Chilli veinal mottle virus
YANG HongKai1(),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
图3. 多基因联合检测体系退火温度的优化
M：DNA分子量标准;1—6：退火温度分别为46、48、50、52、54和56℃;7：阴性对照
Fig. 3. Optimization of the annealing temperature in multi- gene-based PCR detection
Marker DNA (100 bp);1—6：Annealing temperature of 46, 48, 50, 52, 54 and 56℃, respectively;7：Negative control (healthy plant)
