辣椒脉斑驳病毒的多基因联合检测与鉴定
杨宏凯1(
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
Multi-Gene-Based PCR Detection and Identification of Chilli veinal mottle virus
YANG HongKai1(),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
图2. 多基因联合检测RT-PCR结果
M：DNA分子量标准;1：CP单独扩增产物RT-PCR;2：CI单独扩增产物RT-PCR;3：多基因联合检测RT-PCR;4：单独扩增CP的阴性对照;5：单独扩增CI的阴性对照;6：多基因联合检测的阴性对照
Fig. 2. The results of RT-PCR by multi-gene-based PCR detection
Marker DNA (100 bp);1：amplification of CP;2：amplification of CI;3：amplifications by multi-gene-based PCR detection;4：Negative control for amplification of CP (healthy plant);5：Negative control for amplification of CI (healthy plant);6：Negative control by multi-gene-based PCR detection (healthy plant)
