辣椒脉斑驳病毒的多基因联合检测与鉴定
杨宏凯1(
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
),杨晶文1,沈建国2(),蔡伟3,高芳銮1()
Multi-Gene-Based PCR Detection and Identification of Chilli veinal mottle virus
YANG HongKai1(),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
),YANG JingWen1,SHEN JianGuo2(),CAI Wei3,GAO FangLuan1()
图1. 印度进境辣椒常规RT-PCR扩增
M：DNA分子量标准;1：供试辣椒样品CP PCR产物;2—4：阳性对照、阴性对照和空白对照
Fig. 1. Conventional RT-PCR amplification of chilli from India
Marker DNA (100 bp);1：PCR product of CP of ChiVMV isolate from India;2—4：Positive control (with known ChiVMV infected plant), negative control 1 (healthy plant) and negative control 2 (water), respectively
