辣椒脉斑驳病毒的多基因联合检测与鉴定

杨宏凯,杨晶文,沈建国,蔡伟,高芳銮

Multi-Gene-Based PCR Detection and Identification of Chilli veinal mottle virus

YANG HongKai,YANG JingWen,SHEN JianGuo,CAI Wei,GAO FangLuan

表1 本研究中辣椒样品病毒检测所用引物

Table 1 Primers used for the detection of chilli samples in this study

基因 Gene 上下游引物序列 Upper and lower primer sequences (5′-3′) 长度 Length (nt) 退火温度 Tm (℃) 目的片段 Expected size (bp) CP CP861-F: GCAGGAGAGAGTGTTGATGC CP861-R: CAATCCTCGAACGCCCAGCAG CP337-F: ACACCTTCTTGATTATGCTCC CP337-R: ATAAGGCTTCTCAGAATTGCG 20 21 21 21 59.9 63.9 56.1 56.1 861a 337b CI CI655-F: ACAGCRCCAGATGCAAAATC 20 58.0 655 CI655-R: CCTGTGCCYTGYGCATCMA 19 54.1