副猪嗜血杆菌lpxM基因缺失株构建及生物学特性分析
杨君1,2(
),楚品品2,宋帅2,蔡汝健2,杨冬霞2,卞志标2,勾红潮2,李艳2,蒋智勇2,李春玲2(),闫鹤1()
),楚品品2,宋帅2,蔡汝健2,杨冬霞2,卞志标2,勾红潮2,李艳2,蒋智勇2,李春玲2(),闫鹤1()
Construction of lpxM Gene Deletion Strain of Haemophilus parasuis and It's Some Biological Characteristics
YANG Jun1,2(),CHU PinPin2,SONG Shuai2,CAI RuJian2,YANG DongXia2,BIAN ZhiBiao2,GOU HongChao2,LI Yan2,JIANG ZhiYong2,LI ChunLing2(),YAN He1()
),CHU PinPin2,SONG Shuai2,CAI RuJian2,YANG DongXia2,BIAN ZhiBiao2,GOU HongChao2,LI Yan2,JIANG ZhiYong2,LI ChunLing2(),YAN He1()
图3. lpxM基因缺失株的鉴定
M：DL2000 DNA marker;1-3：鉴别缺失片段,分别是缺失株、野生株和阳性对照,引物lpxM-up-EcoRI-F/ lpxM-down-PstI-R;4-6：鉴别卡那抗性表达盒,顺序同上,引物kanR-F/R;7-9：鉴别质粒是否消除,顺序同上,引物sacB-F/R
Fig. 3. Identification of the lpxM gene deletion strain
M: DL2000 DNA marker; 1-3: Identification of the deletion fragments, the deletion strain, wild strain and positive control, primer lpxM-up-EcoRI-F/ lpxM-down-PstI-R ; 4-6: Identification of the kanR resistance expression cassette, the deletion strain ,wild strain and positive control, primer kanR-F/R; 7-9: Identification of plasmid elimination, the deletion strain, wild strain and positive control, primer sacB-F/R
