副猪嗜血杆菌lpxM基因缺失株构建及生物学特性分析
杨君1,2(
),楚品品2,宋帅2,蔡汝健2,杨冬霞2,卞志标2,勾红潮2,李艳2,蒋智勇2,李春玲2(),闫鹤1()
),楚品品2,宋帅2,蔡汝健2,杨冬霞2,卞志标2,勾红潮2,李艳2,蒋智勇2,李春玲2(),闫鹤1()
Construction of lpxM Gene Deletion Strain of Haemophilus parasuis and It's Some Biological Characteristics
YANG Jun1,2(),CHU PinPin2,SONG Shuai2,CAI RuJian2,YANG DongXia2,BIAN ZhiBiao2,GOU HongChao2,LI Yan2,JIANG ZhiYong2,LI ChunLing2(),YAN He1()
),CHU PinPin2,SONG Shuai2,CAI RuJian2,YANG DongXia2,BIAN ZhiBiao2,GOU HongChao2,LI Yan2,JIANG ZhiYong2,LI ChunLing2(),YAN He1()
图1. 重组片段的构建
M：DL2000 DNA ;1：kanR抗性表达盒（909 bp）;2：lpxM-up上游同源臂（489 bp）;3：lpxM-down 下游同源臂（593 bp）;4：M-UKD 重组片段（1991 bp）
Fig. 1. Construction of recombinant fragments
marker;1: kanR resistance expression cassette (909 bp); 2: lpxM-up upstream homology arm (489 bp); 3: lpxM-down downstream homology arm (593 bp); 4: M-UKD recombinant fragment (1991 bp)
