副猪嗜血杆菌lpxM基因缺失株构建及生物学特性分析

杨君,楚品品,宋帅,蔡汝健,杨冬霞,卞志标,勾红潮,李艳,蒋智勇,李春玲,闫鹤

Construction of lpxM Gene Deletion Strain of Haemophilus parasuis and It's Some Biological Characteristics

YANG Jun,CHU PinPin,SONG Shuai,CAI RuJian,YANG DongXia,BIAN ZhiBiao,GOU HongChao,LI Yan,JIANG ZhiYong,LI ChunLing,YAN He

表1 引物序列

Table 1 Primers sequence

引物Primers 序列(5′→3′) Sequence 长度 Length kan-F CATTGCACAAGATAAAAATATAT 909 bp kan-R CAATTAACCAATTCTGATTAG lpxM-up-EcoRI-F CCGGAATTCACCGCTTGT CCTACAACCTGTTTCGCC 489 bp lpxM-up-kan-R TATTTTTATCTTGTGCAATG CAACATTGCCAGGGCTAG lpxM-kan-down -F CTAATCAGAATTGGTTAATTG AGGTCGCCAGCTTATCAC 593 bp lpxM-down-PstI-R TAACTGCAGACAAGCGGT CCGTATTCCGATTTATGTG HPS-F F1：TATCGGGAGATGAAAGAC F2：GTAATGTCTAAGGACTAG 1090 bp HPS-R CCTCGCGGCTTCGTC sacB-F GCAGGAGGCGCAACTCAA 986 bp sacB-R ATTTTAAAGACGTTCGCGC