微小RNA介导东方蜜蜂微孢子虫侵染意大利蜜蜂工蜂的分子机制

耿四海,石彩云,范小雪,王杰,祝智威,蒋海宾,范元婵,陈华枝,杜宇,王心蕊,熊翠玲,郑燕珍,付中民,陈大福,郭睿

The Mechanism Underlying MicroRNAs-Mediated Nosema ceranae Infection to Apis mellifera ligustica Worker

GENG SiHai,SHI CaiYun,FAN XiaoXue,WANG Jie,ZHU ZhiWei,JIANG HaiBin,FAN YuanChan,CHEN HuaZhi,DU Yu,WANG XinRui,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui

表1 本研究使用的引物

Table 1 Primers used in this study

引物ID Primer ID 引物序列 Primer sequence miR-2478-y Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGGTGTC F: GCGCGCGTCGAATCCCACT novel-m0017-5p Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGGGCATA F: GCGCGCGTGGACCAGTGGC novel-m0010-5p Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCTCGCT F: GCGCGCGTTTAGTTTGGCT miR-3968-y Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGGTGTC F: GCGCGCGTCGAATCCCACT miR-146-x Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAACCCAT R: GCGCGCGTGAGAACTGAAT miR-24-y Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGTTCCTG F: GCGCGCGTGGCTCAGTTCA miR-2965-y Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGCTCTC F: GCGCCGGAGGACTGCT miR-318-y Loop: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGACCAAC F: CGGCCGCTCACTGGGC R: CAGTGCGTGTCGTGGAGT 5S rRNA F: CGAGCGGTTTCCCATCTCAGTA R: AAAACACCGGAACTCGTCAGCT