靶向猪内质网应激通路的microRNAs预测与验证
朱静静(
),周晓龙,汪涵,李向臣,赵阿勇,杨松柏()
),周晓龙,汪涵,李向臣,赵阿勇,杨松柏()
Prediction and Verification of MicroRNAs Targeting Porcine Endoplasmic Reticulum Stress Pathway
ZHU JingJing(),ZHOU XiaoLong,WANG Han,LI XiangChen,ZHAO AYong,YANG SongBai()
),ZHOU XiaoLong,WANG Han,LI XiangChen,ZHAO AYong,YANG SongBai()
图3. 双荧光报告基因载体双酶切鉴定
M：DL2000 Marker;1：psiCHECK-2-ATF6-m142-3′UTR,2：psiCHECK- 2-ATF6-m145-3′UTR,3：psiCHECK-2-ATF6-m150-3′UTR,4：psiCHECK- 2-ATF6-m199-3′UTR,5：psiCHECK-2-IRE1-m150-3′UTR,6：psiCHECK- 2-IRE1-m142/145/199-3′UTR,7：psiCHECK-2-PERK-m145/150-3′UTR,8：psiCHECK-2-XBP1-m142/145/150/199-3′UTR,9：psiCHECK-2-GRP78- m145/199-3′UTR
Fig. 3. Identification of luciferase reporter gene vector by XhoⅠ and NotⅠ double digestion
