靶向猪内质网应激通路的microRNAs预测与验证
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朱静静,周晓龙,汪涵,李向臣,赵阿勇,杨松柏
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Prediction and Verification of MicroRNAs Targeting Porcine Endoplasmic Reticulum Stress Pathway
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ZHU JingJing,ZHOU XiaoLong,WANG Han,LI XiangChen,ZHAO AYong,YANG SongBai
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表2 qRT-PCR引物序列
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Table 2 The sequences of qRT-PCR primers
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| 名称 Names | 引物序列 Primer sequence (5′—3′) | 退火温度 Annealing temperature (℃) | 片段长度 Size (bp) | | ATF6 | F:CTAAAGCGAGTCCTCTACG | 60 | 241 | | R:GCCATGCCTGATTTCACA | | | | XBP1 | F:GGATTCTGACGGTGTTGA | 60 | 161 | | R:GGAGGCTGGTAAGGAACT | | | | PERK | F:AGGAGCAAACGGAGCACG | 60 | 187 | | R:GTCGGTGAGGATGAGGATGG | | | | IRE1 | F:GGACTGGCGGGAGAACAT | 60 | 272 | | R:CGTGGTAGTAGGGCTGGAA | | | | GAPDH | F:GGACTCATGACCACGGTCCAT | 60 | 220 | | R:TCAGATCCACAACCGACACGT | | |
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