靶向猪内质网应激通路的microRNAs预测与验证

朱静静,周晓龙,汪涵,李向臣,赵阿勇,杨松柏

Prediction and Verification of MicroRNAs Targeting Porcine Endoplasmic Reticulum Stress Pathway

ZHU JingJing,ZHOU XiaoLong,WANG Han,LI XiangChen,ZHAO AYong,YANG SongBai

表1 双荧光素酶报告基因载体构建引物序列

Table 1 Primers sequences for luciferase reporter gene vector construct

名称 Names 引物序列 Primer sequence (5′—3′) 片段长度 Size (bp) ATF6-m142-3′UTR F:CCGCTCGAGCCGTGGAGTGATTATGTG 413 R:ATTGCGGCCGCCTTCCTGAAACTTTACCCT ATF6-m145-3′UTR F:CCGCTCGAGCCTGGCTTGAATCTTCCC 301 R:ATTGCGGCCGCGTTGTGAGTTCGATCCCT ATF6-m150-3′UTR F:CCGCTCGAGTTTTGTCAGTCCTGGGTC 464 R:ATTGCGGCCGCAAGATACCTTTGTCATTC ATF6-m199a-3′UTR F:CCGCTCGAGTCTTTCTGCCTTCTTGGT 176 R:ATTGCGGCCGCGGAAGATGTAGGGTAATGTG PERK-m145/150-3′UTR F:CCGCTCGAGCATGGAATAGCCCACCTC 777 R:ATTGCGGCCGCTTGGGAAAGTACCGACCT IRE1-m142/145/199-3′UTR F:CCGCTCGAGATCGAACCCGAGCCACTG 1006 R:ATTGCGGCCGCAGACGCCATCATCAATCA IRE1-m150-3′UTR F:CCGCTCGAGAGCCAAGTGCCTTGAGCTG 256 R:ATTGCGGCCGCACAGGCTGACATCAAACAGGA GRP78-m145/199-3′UTR F:CCGCTCGAGACTGCTCTGCTAGTGTTG 349 R:ATTGCGGCCGCACCAGTGTAAATAACAAA XBP1-m142/145/150/199-3′UTR F:CCGCTCGAGCTGCTTTCAACCAGCCACT 596 R:ATTGCGGCCGCCCCTCAGGTAGGCATTCT