葡萄钾离子通道基因VviSKOR的克隆、表达及电生理功能

沈静沅,唐美玲,杨庆山,高雅超,刘万好,程杰山,张洪霞,宋志忠

Cloning, Expression and Electrophysiological Function Analysis of Potassium Channel Gene VviSKOR in Grape

SHEN JingYuan,TANG MeiLing,YANG QingShan,GAO YaChao,LIU WanHao,CHENG JieShan,ZHANG HongXia,SONG ZhiZhong

表1 本研究所用特异性引物

Table 1 Specific primers used in this work

目的 Intention 引物 Primer (5′-3′) 扩增产物大小 Amplicon size (bp) VviSKOR CDS扩增 Amplication of VviSKOR CDS F: ATGATGAATGATTGGTTCTCTG R: CTAAATATCTAGTGTTTCACAT 2385 SKOR特异性表达引物 Specific expression primers of SKOR F: GTGGTTTTCTGTGTGCGACC R: GCCACATGAAGTGGGGTTCT 118 Ubiquitin特异性表达引物 Specific expression primers of Ubiquitin F: CCTCATCTTCGCTGGCAAAC R: GGTGTAGGTCTTCTTCTTGCG 133 pTracer-CMV3-SKOR质粒构建 Construction of plasmid of pTracer-CMV3-SKOR vector F: GAGAGAATTCCATGATGAATGATTGGTTC R: GAGAGCGGCCGCCTAAATATCTAGTGTTTC 2385