),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
图4. GST pull down分析SlVQ6互作蛋白
A：GST pull down分析与SlVQ6相互作用的蛋白质的流程图。将GST-SlVQ6（或GST,阴性对照）添加至GST sheperose 4B beads,将番茄叶片的粗蛋白提取液添加beads,并在4℃孵育过夜。将样品煮沸,用12% SDS-PAGE凝胶分离,回收条带,脱色,干燥,胰蛋白酶消化,并进行LC-MS/MS分析。SBP：特异性结合蛋白;NSBP：非特异性结合蛋白。B：鉴定出37个SlVQ6特异性结合蛋白
Fig. 4. GST pull down analysis of SlVQ6-interacting proteins
A: The flow chart of GST pull down analysis of SlVQ6-interacting proteins. GST-SlVQ6 (or GST, a negative control) was added to GST sheperose 4B beads and crude protein extract of tomato leaves was added to the beads and incubated for overnight at 4℃. The samples were boiled and then separated by 12% SDS-PAGE gels. The bands were cut out, destained, dried, digested using trypsin, and the LC-MS/MS analysis was performed. SBP: Specific binding proteins; NSBP: Non-specific binding proteins. B: Thirty-seven of SlVQ6-specific binding proteins were identified
