应用GST pull-down技术筛选番茄SIVQ6互作蛋白
原贵波(
),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
Screening of Interacting Protein of Tomato SIVQ6 by GST Pull-Down
YUAN GuiBo(),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
图3. SlMPK1磷酸化纯化的SlVQ6
A：His-SlMPK1和GST-SlVQ6用于带有32P标记的磷酸化反应,GST用作阴性对照,单独添加GST-SlVQ6证明了GST-SlVQ6的自磷酸化反应;B：内参参照考马斯亮蓝染色
Fig. 3. In-gel analysis of phosphorylation of purified SlVQ6 by SlMPK1
A: His-tagged SlMPK1 and GST-tagged SlVQ6 were used in phosphorylation reactions with 32P labeling, GST was used as a negative control, and the autophosphorylation of GST-SlVQ6 was proved by adding GST-SlVQ6 alone; B: Coomassie blue staining in the below panel
