应用GST pull-down技术筛选番茄SIVQ6互作蛋白
原贵波(
),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
Screening of Interacting Protein of Tomato SIVQ6 by GST Pull-Down
YUAN GuiBo(),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
图2. 融合蛋白的表达纯化
A：SDS-PAGE分析Arctic Express中的表达形式,M：Marker;1：未被诱导;2：诱导;3：0.5 mmol·L-1 IPTG诱导上清液;4：0.5 mmol·L-1 IPTG诱导沉淀;箭头：GST-SlVQ6蛋白。B：融合蛋白纯化的SDS-PAGE,M：Marker;1：未被纯化;2：流出液;3：洗脱液;箭头：GST-SlVQ6蛋白
Fig. 2. Expression and purification of fusion protein
A: SDS-PAGE of expression form analysis in Arctic Express, M: Marker; 1: Un-induced; 2: Induced; 3: Supernatant of induction with 0.5 mmol·L-1 IPTG; 4: Precipitate of induction with 0.5 mmol·L-1 IPTG; Arrow: GST-SlVQ6 protein. B: SDS-PAGE of fusion protein purification, M: Marker; 1: Un-purified; 2: Flow through; 3: Elution; Arrow: GST-SlVQ6 protein
