应用GST pull-down技术筛选番茄SIVQ6互作蛋白
原贵波(
),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
),莫双榕,钱莹,臧栋楠,杨帆,蒋红亮,武媛,丁海东()
Screening of Interacting Protein of Tomato SIVQ6 by GST Pull-Down
YUAN GuiBo(),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
),MO ShuangRong,QIAN Ying,ZANG DongNan,YANG Fan,JIANG HongLiang,WU Yuan,DING HaiDong()
图1. 融合表达载体的构建
A：重组质粒pGEX-4T-1-PC-SlVQ6的双酶切鉴定,M：Marker;1：酶切前质粒;2：酶切后质粒。B：pGEX-4T-1-PC-SlVQ6的部分序列比对
Fig. 1. Construction of fusion expression vector
A: Double enzyme digestion of recombinant plasmid pGEX-4T-1-PC-SlVQ6, M: Marker; 1: Pre-digested plasmid; 2: Plasmid after digestion. B: The partial sequence alignment of pGEX-4T-1-PC-SlVQ6
