靶向测序基因型检测（GBTS）技术及其应用

徐云碧,杨泉女,郑洪建,许彦芬,桑志勤,郭子锋,彭海,张丛,蓝昊发,王蕴波,吴坤生,陶家军,张嘉楠

Genotyping by Target Sequencing (GBTS) and Its Applications

XU Yunbi,YANG QuanNü,ZHENG HongJian,XU YanFen,SANG ZhiQin,GUO ZiFeng,PENG Hai,ZHANG Cong,LAN HaoFa,WANG YunBo,WU KunSheng,TAO JiaJun,ZHANG JiaNan

表2 靶向测序基因型检测（GBTS）的应用

Table 2 Applications of Genotyping by Target Sequencing (GBTS)

应用领域 Applications 40K+ 30K 20K 10K 5K 1K <200 生物进化Biological evolution +++ +++ ++ ++ + 种质资源评价Germplasm evaluation +++ +++ ++ ++ + + 分类Classification +++ +++ +++ +++ ++ ++ 图谱构建Linkage map construction +++ +++ +++ ++ + + 基因定位和克隆Gene mapping/cloning +++ +++ +++ +++ ++ + 标记-性状关联Marker-trait association +++ ++ ++ + 后裔测验Progeny testing +++ +++ +++ +++ +++ +++ +++ 基因渐渗Gene introgression +++ +++ +++ +++ +++ ++ + 基因累加Gene pyramiding +++ +++ +++ +++ ++ + + 品种权保护Variety right protection +++ +++ +++ +++ ++ ++ + 质量控制Quality control +++ +++ +++ +++ ++ ++ + 生物检测Bioassay +++ +++ +++ +++ ++ + +