野猪粪便中乳酸菌的分离鉴定及特性研究
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王翌,李淼,李永锋,孙元,仇华吉
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Identification and Properties of Lactic Acid Bacteria Isolated from Wild Boar Feces
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WANG Yi,LI Miao,LI YongFeng,SUN Yuan,QIU HuaJi
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表1 PCR扩增引物
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Table 1 The primer sequences for PCR amplification
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| 基因名称 Gene | 引物序列 (5°-3°)
Primer sequence (5°-3°) | 产物长度
Length (bp) | | 16S rDNA | 27F: AGAGTTTGATCCATGGCTCAG | 1514 | | 1541R: AAGGAGGTGATCCAGCC | | ace | F: CAGGCCAACATCAAGCAACA | 125 | | R: GCTTGCCTCGCCTTCTACAA | | | agg | F: AAGAAAAAGAAGTAGACCAAC | 1553 | | R: AAACGGCAAGACAAGTAAATA | | | atpA | F: CCAGGTCGTGAAGCTTATCC | 110 | | R: GGTAAGGCCGTCATTGAACC | | | cfa1 | F: ACGACCTGTTGTTCGACCTG | 150 | | R: ACGACCTGTTGTTCGACCTG | | | ebp | F: AATGTGTTAAACCATCAAGGGAAT | 372 | | R: ACTCCTTTTTGAACTTCACCAATC | | | espA | F: TTTGGGGCAACTGGAATAGT | 407 | | R: CCCAGCAAATAGTCCATCAT | | | gelE | F: CGAAGTTGGAAAAGGAGGC | 372 | | R: GGTGAAGAAGTTACTCTGA | | | groEL | F: GTTTGATCGCGGCTATCTGA | 150 | | R: CCTTGTTGMACGATTTCTTG | | | hisD | F: TGAACCACTCGGTGACTACG | 150 | | R: GGAGCTTCCTTAGCCAAAGC | | | mleS | F: ACAAGGTCTCAGCGTTCAGC | 140 | | R: GACTGGGATTCCAGCTGATG | | | sprE | F: GGTAAACCAACCAAGTGAATC | 300 | | R: R: TTCTTCCGATTGACGCAAAA | |
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