棉花黄萎病菌VdHP1的克隆及功能分析
孙琦(
),何芳,邵胜楠,刘政(),黄家风()
),何芳,邵胜楠,刘政(),黄家风()
Cloning and Functional Analysis of VdHP1 in Verticillium dahliae from Cotton
SUN Qi(),HE Fang,SHAO ShengNan,LIU Zheng(),HUANG JiaFeng()
),HE Fang,SHAO ShengNan,LIU Zheng(),HUANG JiaFeng()
图6. VdHP1基因敲除对大丽轮枝菌致病力的影响
A：感病棉花的症状,照片拍摄于接种后20 d ;B：棉花发病后的病情指数;C：感病棉花维管束变褐现象;D：棉花根、茎、叶中的真菌生物量;E：与侵染钉形成和分泌蛋白释放相关基因在VdHP1基因敲除突变体中的相对表达量;F：玻璃纸穿透试验
Fig. 6. Effect of VdHP1 gene knockout on pathogenicity in V. dahliae
A：The symptoms of diseased cotton plants, the photograph were taken at 20βdays post inoculation;B：Disease index of cotton plants;C：Vascular discoloration of diseased cotton plants;D：Relative quantification of fungal biomass in roots, stems and leaves of cotton plants;E： The transcriptional expression of genes involved in penetration peg formation and delivery of secretory protein in VdHP1 gene knockout mutants;F：Cellophane penetration test of VdHP1 gene knockout mutants
