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棉花黄萎病菌VdHP1的克隆及功能分析
孙琦(),何芳,邵胜楠,刘政(),黄家风()
Cloning and Functional Analysis of VdHP1 in Verticillium dahliae from Cotton
SUN Qi(),HE Fang,SHAO ShengNan,LIU Zheng(),HUANG JiaFeng()

图2. 大丽轮枝菌V592菌株VdHP1基因的敲除
A:VdHP1基因敲除载体构建图;B:利用特异引物对目标基因VdHP1进行PCR检测;C:利用RT-qPCR对VdHP1的转录水平进行确定

Fig. 2. Knockout of VdHP1 in V. dahliae V592 strain。。。
A:Strategy diagram of VdHP1 knockout vector construction;B:Specific primers were used to detect VdHP1 by PCR;C:Confirmation of transcriptional expression of VdHP1 by RT-qPCR analysis