棉花黄萎病菌VdHP1的克隆及功能分析

孙琦,何芳,邵胜楠,刘政,黄家风

Cloning and Functional Analysis of VdHP1 in Verticillium dahliae from Cotton

SUN Qi,HE Fang,SHAO ShengNan,LIU Zheng,HUANG JiaFeng

表2 被检测的致病相关基因及其引物

Table 2 The genes involved in pathogenicity and primers used for RT-qPCR

基因Gene 引物Primer 引物序列Primer sequence (5′-3′) VdPKS1 (VDAG_00190) VdPKS1-F ATGGTCGGCACCATGTCTTTTCTCC VdPKS1-R GCCTGTTCGAGAAAGGTCTTGGCAA VMK1 (VDAG_09461.1) VMK1-F CGCAGCAACGCCCCTAATC VMK1-R GGCAGTGGTCATCGGAGAGGT VdNLP1 (VDAG_04701.1) VdNLP1-F TCGGTCTTTGCCCTCGTC VdNLP1-R GCCTGGTTTGCGTTGTTC VdNLP2 (VDAG_01995.1) VdNLP2-F AAGCCGTACCTCAAGGTGTTCA VdNLP2-R CCGACCCAAAGTCCGTGTTCT VdCYC8 (VDAG_07052) VdCYC8-F GGATGCCCTCGATGCTTACT VdCYC8-R CGTCGCTGATCTGGTTGTTG VGB (JQ665433.1) VGB-F GCAATCTCCAAACGACGTGTCG VGB-R GCGAACTGACGTGTGGTGTCGG VdSge1 (VDAG_06298.1) VdSge1-F CATGGATCCTTCCGAGGCATCTAG VdSge1-R GATGATGCGGGACGCTTCTGAAC VdHog1 (VDAG_08982) VdHog1-F CTTCCACGTGTCTACTGGCAGG VdHog1-R TGCTCCTTACCACGACCTTACCGA VdNoxB (VDAG_09930) VdNoxB-F TGCGTGGCAAGCATAAGACATAC VdNoxB-R GACAGCACGAGTGAAATCACCAAC VdPls1 (VDAG_01769) VdPls1-F ATGGTCAACAAGATCCTCGCGA VdPls1-R TCCGGCTGCTCAAACATGTTGT VdSep5 (VDAG_04382) VdSep5-F AGCTCGACCTGGACGAGGA VdSep5-R GAGGCTTCGTTATCAATCTCGTCTC VdSho1 (VDAG_01836) VdSho1-F GAGATAACCCAAAGGGCCATGGG VdSho1-R GAGAGCGTATCCAATCGCACC VdCrz1 (VDAG_03208) VdCrz1-F ATGGATCAGCAAGCTCAACATCG VdCrz1-R GATCCAGACCGAGACCGAGAC VdLAC (VDAG_00189) VdLAC-F ATGCTCTTCTCGCGTTTCCTCA VdLAC-R GCCACTGACCATTGATGCCAAT VdCmr1 (VDAG_00195) VdCmr1-F GCGCCACAAGCTCTGCATCTTC VdCmr1-R CAGAATCAAGGTGGCGCGATACAC VdPLP (VDAG_00942) VdPLP-F GCTGACCAGTATCTGTCGGAGG VdPLP-R ATGACGACTGGCTTCTCGGCCT Vdpf (VDAG_08521.1) Vdpf-F ACCATTTTCAACAGTCGGGTACGCG Vdpf-R GTGTGACGTACCAGCAACCGCTT β-tubulin (DQ266153) β-tubulin-F TCACCAGCCGTGGCAAGGTTG β-tubulin-R AGCAAAGGGCGGTCTGGACGTTG