棉花黄萎病菌VdHP1的克隆及功能分析

孙琦,何芳,邵胜楠,刘政,黄家风

Cloning and Functional Analysis of VdHP1 in Verticillium dahliae from Cotton

SUN Qi,HE Fang,SHAO ShengNan,LIU Zheng,HUANG JiaFeng

表1 载体构建所用引物

Table 1 Primers used for vector construction

引物Primer 引物序列Primer sequence (5′-3′) VdHP1 full-F ATGCGTTTCTTCGCCTTTTT VdHP1 full-R TAGTCCATTCTGATCCATGT VdHP1 up-F CTTGCTGAGGTCTTAATTAAACCTCCAAGGCATCGTTGC VdHP1 up- R AGTGCTGAGGCATTAATTAACAAACGAGACGCGAATGGTG VdHP1 down-F CCCGCTGAGGACTTAATTAA GGATCTTGCGTCTCGTAGGT VdHP1 down- R CTCGCTGAGGGTTTAATTAA AGGCCATTCATTACGATGCC HygU AACCACGGCCTCCAGAAGAA HygL AGCCTGACCTATTGCATCTCCC ECVdHP1-F CGGCCAGTGCCAAGCTT AAATATCGTGTGGTGCGAAA ECVdHP1-R GCAGCTTCTGCGAATTC TTAGTCCATTCTGATCCATG OEVdHP1-F AATGAATATAGGCCGTCGACATGCGTTTCTTCGCCTTTTT OEVdHP1-R CTGCATCCGAATTCACTAGTTTAGTCCATTCTGATCCATGC VdHP1-qPCR-F AGAGCCAGAGGGTTCGTGGA VdHP1-qPCR-R ATGCGTTTCTTCGCCTTTTTACAGA