茶树CsWRKYIIcs转录因子的克隆及功能分析

肖罗丹,唐磊,王伟东,高岳芳,黄伊凡,孟阳,杨亚军,肖斌

Cloning and Functional Analysis of CsWRKYIIcs Transcription Factors in Tea Plant

XIAO LuoDan,TANG Lei,WANG WeiDong,GAO YueFang,HUANG YiFan,MENG Yang,YANG YaJun,XIAO Bin

表1 克隆、荧光定量和构建酵母载体引物

Table 1 Primers of cloning, qRT-PCR and constructing yeast vectors

引物 Primer 上游序列（5′-3′） Upstream primer 下游序列（5′-3′） Downstream primer 作用 Function CsWRKYIIc1 ATC CTT CCA AAC GAT GAC ATT ACCATTGTAAGTTGTACACATGG 克隆 Cloning CsWRKYIIc2 TGAAGAAGAATCTGTGCTCCT TCTAAAGATAACCCAACAAACCTC CsWRKYIIc3 AGAAACTGCACTTGAAGGAGCT CTCAACCTCAAGACCAGTCAGT CsWRKYIIc4 TGATGTAGAAGAAATTGGAGTTGTG ACATACCAGTAATTGGGTTTCCA CsWRKYIIc5 TGAGATAACGCGTAGTCCCAATAG CTGTGGTGAATTAGTTTAGTGCAT CsWRKYIIc6 CGTGGTGGCTACAGAGACAT GTACTTACGTGAGACTGGTTAGCC CsWRKYIIc7 TTCATACTTTGCTCTCTCCCTT TGAGTTGTTCTTGTACAAAGAGGT CsWRKYIIc8 AGAGACCTTAGACAAATCTTCCTGT ATAAAATTAGCAATTGAAAGGGGCT CsWRKYIIc9 TGACCTTAGAGTCAAACCTATCT TATGAATTGTCCCATACCTGACT qRT- CsWRKYIIc1 GAGGAAATATGGACAGAAAGCTG GCATTCCTTCGTAAGTTGTCAC 荧光定量qRT-PCR qRT- CsWRKYIIc2 GAATCATGCCAGAAAGTGCC GGTGTTGGGTTGTGAAAATAGG qRT- CsWRKYIIc3 TCCACAACCTCAATTCCAACCG AGCAAAGACGATGACAGTGA qRT- CsWRKYIIc4 ACCACAATCTCAACCTCAACC CGACACAATGACAGAGCAAAAG qRT- CsWRKYIIc5 CAGCATTGTCACCATACAGTTG AGGCAGCTTCATGATTGACTAG qRT- CsWRKYIIc6 AAGCCACTTGACTCCTTCAG TGGGAAGTTGAGGATTTGGC qRT- CsWRKYIIc7 TTCGGGTTCATGGACTTACTG AGTCATGGGCTGGTTATTCAAG qRT- CsWRKYIIc8 AGGTCGTTTGAAGATCCATCAG CCACATTACCCCTCAGAGTTG qRT- CsWRKYIIc6 TCGATCTCTTCCTCGTCTACTG CCTCCATCTTCCACCTCTTTT qRT- CsWRKYIIc7 GCCATCTTTGATTGGAATGG GGTGCCACAACCTTGATCTT qRT- CsWRKYIIc8 AGGTCGTTTGAAGATCCATCAG CCACATTACCCCTCAGAGTTG qRT- CsWRKYIIc9 TCGATCTCTTCCTCGTCTACTG CCTCCATCTTCCACCTCTTTT qRT- Csβ-actin GCCATCTTTGATTGGAATGG GGTGCCACAACCTTGATCTT pGBKT7-CsWRKYIIc1 TCAGAGGAGGACCTGCATATGATGGACAACTACTCATCAAT CGGCCTCCATGGCCATATGAAATGCCGTGTAAATTTG 构建酵母载体 Constructing yeast vectors pGBKT7-CsWRKYIIc2 TCAGAGGAGGACCTGCATATGATGGAGAAGAAGAAACAGGA CGGCCTCCATGGCCATATGCTCTTCTTTTGGCTCCTT pGBKT7-CsWRKYIIc3 TCAGAGGAGGACCTGCATATGATGCTTGTTGTTGTGAGTGA CGGCCTCCATGGCCATATGCTCGGGTTTTGCCTCCTT pGBKT7-CsWRKYIIc4 TCAGAGGAGGACCTGCATATGATGGAGAGTAAAGAAGCTGT CGGCCTCCATGGCCATATGGTCATCCTTTGTAACAAG pGBKT7-CsWRKYIIc5 TCAGAGGAGGACCTGCATATGATGGATGAGAACGACAGAGT CGGCCTCCATGGCCATATGTTGATTGCGCATTCCAGG pGBKT7-CsWRKYIIc6 TCAGAGGAGGACCTGCATATGATGGATGATGATGATAAGGA CGGCCTCCATGGCCATATGCTGATTGCGCATTCCAGG pGBKT7-CsWRKYIIc7 TCAGAGGAGGACCTGCATATGATGGAGAGGAAACAAGCTGT CGGCCTCCATGGCCATATGTTTGATGAGCCAAATTTC pGBKT7-CsWRKYIIc8 TCAGAGGAGGACCTGCATATGATGTCTGATGAACACAGAGA CGGCCTCCATGGCCATATGTGGCTCTTGTTTAAGAAA pGBKT7-CsWRKYIIc9 TCAGAGGAGGACCTGCATATGATGTCTGATGAACCAGGAGG CGGCCTCCATGGCCATATGTGGCTCTTGTTTAAAAAA