一种CRISPR-Cas9介导的拟南芥高效基因编辑系统的构建与应用
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张成,何明亮,汪威,徐芳森
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Development of an Efficient Editing System in Arabidopsis by CRISPR-Cas9
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ZHANG Cheng,HE MingLiang,WANG Wei,XU FangSen
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表1 TCH4 CRISPR T1代植株的编辑类型及比例
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Table 1 Mutation patterns and proportions TCH4 edited T1 plants
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T1代植株染色体的编辑类型
Mutation patterns of chromosome in T1 plants | 发生次数
No. of edited lines | 突变比例
Mutation rate (%) | | 无None | 18 | 15.25 | | +1 | 45 | 38.14 | | +32 | 1 | 0.85 | | -1 | 11 | 9.32 | | -2 | 4 | 3.39 | | -4 | 1 | 0.85 | | -5 | 2 | 1.69 | | -6 | 2 | 1.69 | | -7 | 4 | 3.39 | | -10 | 4 | 3.39 | | -11 | 1 | 0.85 | | -12 | 1 | 0.85 | | -13 | 3 | 2.54 | | -14 | 1 | 0.85 | | -16 | 1 | 0.85 | | -17 | 1 | 0.85 | | -19 | 1 | 0.85 | | -20 | 6 | 5.08 | | -22 | 1 | 0.85 | | -23 | 1 | 0.85 | | -31 | 1 | 0.85 | | -34 | 2 | 1.69 | | -49 | 1 | 0.85 | | -55 | 1 | 0.85 | | -218 | 1 | 0.85 | | -313 | 1 | 0.85 | | -503 | 1 | 0.85 | | -577 | 1 | 0.85 | | 合计Total | 118 | 100.00 |
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