基于PTN系统分析不同种植地转基因水稻种子 可培养内生真菌菌群的多样性

赵艳,王天圻,朱军莉

Diversity of Endophytic Fungi in Transgenic Rice Seeds from Different Planting Sites Based on PTN System

ZHAO Yan,WANG TianQi,ZHU JunLi

表1 水稻种子可培养内生真菌的鉴定结果

Table 1 Identification results of culturable endophytic fungi in rice seeds

编号 Number 纲 Class 目 Order 科 Family 属 Genera 比对相似种 Compare similar species 覆盖率 Coverage (%) 相似度 Similarity (%) 比对结果 Comparison result IR (%) WF6 Ascomycetes Pleosporales Pleosporaceae Curvularia Curvularia lunata strain S3-2 97 99 Curvularia lunata strain WF6 0.54 WF44 Curvularia lunata isolate E16 97 99 Curvularia lunata isolate WF44 0.08 合计Total 0.63 WF17 Pleosporales Pleosporales sp. strain APBSDSF36 97 100 Pleosporales sp. strain WF17 0.04 合计Total 0.04 WF22 Phaeosphaeria Phaeosphaeriopsis musae isolate DFFSCS029 99 99 Phaeosphaeriopsis musae strain WF22 0.04 WF55 Phaeosphaeria papayae isolate LQ122404 98 99 Phaeosphaeria papayae isolate WF55 0.04 合计Total 0.08 WF40 Setophoma Setophoma vernoniae strain CPC 23123 92 93 Setophoma vernoniae strain .WF40 0.13 合计Total 0.13 WF39 Ascomycetes Pleosporales Pleosporaceae Ophiosphaerella Ophiosphaerella agrostidis strain MFLUCC 11-0152 95 99 Ophiosphaerella agrostidis strain.WF39 0.13 WF58 Ophiosphaerella agrostidis isolate MFLUCC 16-0895 94 99 Ophiosphaerella agrostidis isolate WF58 0.04 合计Total 0.17 WF41 Dendryphiella Dendryphiella sp. crri.34 98 99 Dendryphiella sp. WF41 0.83 WF42 Dendryphiella sp. crri.34 97 99 Dendryphiella sp. WF42 0.75 WF45 Dendryphiella sp. crri.34 98 99 Dendryphiella sp.WF45 0.08 WF46 Dendryphiella sp. crri.34 98 99 Dendryphiella sp.WF46 0.08 WF59 Dendryphiella sp. crri.34 98 99 Dendryphiella sp. WF59 0.13 合计Total 1.88 WF48 Bipolaris Bipolaris oryzae isolate BO1 97 99 Bipolaris oryzae isolate WF48 0.04 WF62 Bipolaris sorokiniana strain A0606 95 97 Bipolaris sorokiniana strain WF62 0.04 合计Total 0.08 WF49 Pithomyces Leptosphaerulina chartarum strain DH08111quan1 98 99 Leptosphaerulina chartarum strain WF49 0.04 合计Total 0.04 WF43 Didymellaceae Epicoccum Epicoccum sorghinum strain BJ-F1 97 99 Epicoccum sorghinum strain.WF43 0.08 WF47 Epicoccum sorghinum isolate M3 98 99 Epicoccum sorghinum isolate WF47 0.13 WF52 Epicoccum sorghinum isolate M3 97 99 Epicoccum sorghinum isolate WF52 0.38 WF53 Epicoccum sorghinum isolate M3 96 99 Epicoccum sorghinum isolate WF53 0.04 合计Total 0.63 WF51 Leptosphaeriaceae Leptosphaeria Leptosphaeria sp. SL14 99 99 Leptosphaeria sp. WF51 0.04 WF61 Leptosphaeria sp. isolate LQ122416 98 99 Leptosphaeria sp. isolate WF61 0.04 WF64 Leptosphaeria sp. isolate LQ122417 98 99 Leptosphaeria sp. isolate WF64 0.04 合计Total 0.13 WF20 Moniliales Dematiaceae Nigrospora Nigrospora sp. JL4-CGL24 99 99 Nigrospora sp. WF20 0.04 WF57 Nigrospora sp. HCH267 98 99 Nigrospora sp. WF57 0.04 WF60 Nigrospora oryzae strain APBSMLF76 98 99 Nigrospora oryzae strain WF60 0.04 合计Total 0.13 WF3 Cladosporium Cladosporium oxysporum isolate DFFSCS018 100 99 Cladosporium sp. WF3 0.04 WF19 Cladosporium oxysporum strain SCAU101 99 99 Cladosporium oxysporum strain WF19 0.04 WF63 Cladosporium xanthochromaticum 98 99 Cladosporium xanthochromaticum WF63 0.08 WF65 Cladosporium tenuissimum strain APBSDSF6 99 99 Cladosporium tenuissimum strain WF65 0.04 合计Total 0.21 WF4 Alternaria Alternaria sp. H6_E08_1104035148Q 100 99 Alternaria sp.WF4 0.04 WF18 Alternaria alternata strain EN24 98 100 Alternaria alternata strain WF18 0.04 WF31 Alternaria longissima strain CLB44 98 99 Alternaria longissima strain WF31 0.04 合计Total 0.13 WF9 Tuberculariaceae Fusarium Fusarium chlamydosporum NBAIM:236 98 100 Fusarium chlamydosporum strain WF9 0.04 WF14 Fusarium chlamydosporum isolate LrSF22 99 99 Fusarium chlamydosporum strain WF14 0.04 WF15 Fusarium sp. AL-14 IRH-2012a 98 99 Fusarium sp. WF15 0.04 WF16 Fusarium equiseti strain CP16 99 99 Fusarium equiseti strain WF16 0.04 WF38 Fusarium sp. Z13-09 96 99 Fusarium sp.WF38 0.08 WF50 Fusarium sp. AL-15 IRH-2012b 98 99 Fusarium sp.WF50 0.38 WF54 Fusarium proliferatum isolate MC-23-F 99 99 Fusarium proliferatum isolate WF54 0.04 WF56 Fusarium proliferatum isolate 10R-7 98 99 Fusarium proliferatum isolate WF56 0.04 WF66 Fusarium proliferatum isolate LrBF18 99 99 Fusarium proliferatum isolate WF66 0.04 合计Total 0.75 WF23 Fungi Imperfecti Hypocreales Hypocreaceae Letendraea helminthicola Letendraea helminthicola strain B1A0062SNA2CC1081 88 100 Letendraea helminthicola strain WF23 0.04 合计Total 0.04 总分离率Total IR(%) 5.04