IGF-1调控RBM3表达抑制低温应激诱导牦牛卵丘细胞凋亡

潘阳阳,王萌,芮弦,王立斌,何翃闳,王靖雷,马睿,徐庚全,崔燕,樊江峰,余四九

RNA-Binding Motif Protein 3(RBM3) Expression is Regulated by Insulin-Like Growth Factor (IGF-1) for Protecting Yak (Bos grunniens) Cumulus Cells from Apoptosis During Hypothermia Stress

PAN YangYang,WANG Meng,RUI Xian,WANG LiBin,HE HongHong,WANG JingLei,MA Rui,XU GengQuan,CUI Yan,FAN JiangFeng,YU SiJiu

表1 Real-time PCR引物信息

Table 1 The information of primers used in Real-time PCR

基因 Gene 引物序列 Primer sequence 温度 Tm (℃) 产物大小 Product size (bp) GenBank 登录号 GenBank accession No. RBM3 F:GCTTCATCACCTTCACCAATC R:GGTATGACAATCGACCTGGAGC 60 220 MF142258.1 Bax F:TTTGCTTCAGGGTTTCATC R:CAGCTGCGATCATCCTCT 59 174 NM173894.1 Bcl-2 F:CTGCACCTGACGCCCTTCAC R:GCGTCCCAGCCTCCGTTGT 62 236 NM001166486.1 β-actin F:CTTCAACACCCCTGCCAT R:CTCGGCTGTGGTGGTGAAG 60 238 JF830811