飞蝗核受体基因LmE75的分子特性和功能分析

刘晓健,郭俊,张学尧,马恩波,张建珍

Molecular Characteristics and Function Analysis of Nuclear Receptor Gene LmE75 in Locusta migratoria

LIU XiaoJian,GUO Jun,ZHANG XueYao,MA EnBo,ZHANG JianZhen

表2 所用RT-qPCR和RNAi的引物信息

Table 2 Primers used for RT-qPCR and RNAi analysis

基因Gene 引物序列 Primer sequence (5′-3′) 用途 Application 产物长度 Length of product (bp) β-actin-F/R CGAAGCACAGTCAAAGAGAGGTA/GCTTCAGTCAAGAGAACAGGATG RT-qPCR 156 LmE75A-F/R TTCCGTGTCCTCCAAGCTAG/ACCTTGCACGGGATGATCTC 111 LmE75B-F/R ATGATCCTGTCCGACGGC/CCCGTCGAACTCTATGTGGA 150 LmE75C-F/R ATGACTGTGAAGGACCCGG/ACTCTTCTAGAACCCTGCCT 133 LmE75-F/R CTGCTCGGTGGTGGTGAT/GTCATCTTGAAGGCGAGCAG 85 LmEcR-F/R GACAAACTGCTACGGGAAGA/CTATCGTTTCTCCCATACCAG 172 dsLmE75-F/R taatacgactcactatagggAGCTGGAGGACCAGTCTGC/taatacgactcactatagggGCGAAGTCGAACATGGAGTC RNAi 460 dsLmEcR-F/R taatacgactcactatagggCAGCAACGCCGCACCCT/taatacgactcactatagggCACTGGTACACGGCATTT 408 dsGFP-F/R taatacgactcactatagggTGGAGAGGGTGAAGG/taatacgactcactatagggGGCAGATTGTGTGGAC 712