甘薯羽状斑驳病毒O株系和RC株系中国分离物全基因组 序列分析及其遗传特征
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秦艳红,王永江,王爽,乔奇,田雨婷,张德胜,张振臣
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Complete Nucleotide Sequence Analysis and Genetic Characterization of the Sweet potato feathery mottle virus O and RC Strains Isolated from China
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QIN YanHong,WANG YongJiang,WANG Shuang,QIAO Qi,TIAN YuTing,ZHANG DeSheng,ZHANG ZhenChen
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表2 引物序列及目的片段大小
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Table 2 Primer sequences and expected size of the PCR product for each primer pair
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扩增片段
Amplified fragment | 引物名称
Primer name | 引物序列
Primer sequence (5′→3′) | 目的片段大小
Expected size (bp) | | O-1/RC-1 | 1-F | AATAACACAACWCAAYACAACAYAASAAAACT | 4784/4711 | | 4711-R | GTGATATCATCTAARCTYTGATG | | O-2 | 4689-F | CATCARAGYTTAGATGATATCAC | 5692 | | 10380-R | CATATCGCGCAAGACTCATATC | | O-3 | SPFMV-O-4160-F | AAGCGACAGAGCAACAGACTTATGTACTA | 1124 | | SPFMV-O-5284-R | GATATTGCGTTGTAAATTCAACCTCACGTC | | RC-2 | SPFMV-RC-4260-F | AAGTTTGTGAATATGCTACTAGTCATCA | 3814 | | SPFMV-RC-8220-R | TACCTTCTAGGTCTGTGATCAGTTTAGAC | | RC-3 | SPFMV-RC-7580-F | CCGATAGCTAGCGTCATATGCCATCTCG | 3426 | | SPFMV-11000-R | TTGGCTCGATCACGAACCAAAAAGGCT |
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