公主岭霉素诱导对育苗期水稻耐冷性的影响

安俊霞,赵宇,张正坤,史海鹏,纪东铭,曹洪翼,杜茜,李启云

Induction of Cold Tolerance in Rice at the Breeding Stage by Gongzhulingmycin

AN JunXia,ZHAO Yu,ZHANG ZhengKun,SHI HaiPeng,JI DongMing,CAO HongYi,DU Qian,LI QiYun

表1 4个耐冷相关基因RT-qPCR的引物

Table 1 Primers of 4 cold tolerance genes for RT-qPCR

基因 Gene 功能 Function 引物序列 Primer sequence OsNAC6[24] 转录因子Transcription factor F: 5′TCCGCCCGCAAGAGAACAG3′ R: 5′ACCCCCACCATCGGCTTCCT3′ OsSADMC[2] S-腺苷甲硫氨酸脱羧酶S-adenosylmethionine decarboylase F: 5′ATGGTGAACCCTGCTCCT3′ R: 5′GCAGAAGGCAAAGGAAAT3′ OsETR4[25] 乙烯受体Ethylene receptor F: 5′GTCTTCCACCTGCTGCTTG3′ R: 5′CCTCTCTTCACCCACATTGC3′ OsZFP151[26] C2H2型锌指蛋白C2H2 zinc finger protein F: 5′GCTCTCCATCAGTTGCTCCT3′ R: 5′CTTCATCATCCCACACCAGA3′ OsActin[27] 内参基因Internal reference gene F: 5′CCTGGCAGTATGAAGGTAGTTG3′ R: 5′GAAGCACTTCATGTGGACGAT3′