东方蜜蜂微孢子虫孢子中长链非编码RNA的竞争性内源RNA调控网络及潜在功能
周丁丁,史小玉,王杰,范元婵,祝智威,蒋海宾,范小雪,熊翠玲,郑燕珍,付中民,徐国钧,陈大福,郭睿()
Investigation of Competing Endogenous RNA Regulatory Network and Putative Function of Long Non-Coding RNAs in Nosema ceranae Spore
ZHOU DingDing,SHI XiaoYu,WANG Jie,FAN YuanChan,ZHU ZhiWei,JIANG HaiBin,FAN XiaoXue,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,XU GuoJun,CHEN DaFu,GUO Rui()

图5. 东方蜜蜂微孢子虫孢子中lncRNA、靶miRNA和靶mRNA的RT-PCR验证
M:DNA marker;1:假定蛋白NCER_101243编码基因Hypothetical protein NCER_101243 coding gene;2:假定蛋白NCER_102113编码基因Hypothetical protein NCER_102113 coding gene;3:假定蛋白NCER_100958编码基因Hypothetical protein NCER_100958 coding gene;4:假定蛋白NCER_101315编码基因Hypothetical protein NCER_101315 coding gene;5:假定蛋白NCER_101322编码基因Hypothetical protein NCER_101322 coding gene;6:假定蛋白NCER_101544编码基因Hypothetical protein NCER_101544 coding gene;7:假定蛋白NCER_100991编码基因Hypothetical protein NCER_100991 coding gene;8:假定蛋白NCER_1011114编码基因Hypothetical protein NCER_101114 coding gene;9:MSTRG.3636.1;10:MSTRG.4883.1;11:nce-miR-8565;12:nce-miR-7502;13:nce-miR-7729;N1、N2:阴性对照(无菌水)Negative control (sterile water);N3:阴性对照(DEPC水)Negative control (DEPC water)

Fig. 5. RT-PCR verification of lncRNAs, target miRNAs and target mRNAs in N. ceranae spore