东方蜜蜂微孢子虫孢子中长链非编码RNA的竞争性内源RNA调控网络及潜在功能

周丁丁,史小玉,王杰,范元婵,祝智威,蒋海宾,范小雪,熊翠玲,郑燕珍,付中民,徐国钧,陈大福,郭睿

Investigation of Competing Endogenous RNA Regulatory Network and Putative Function of Long Non-Coding RNAs in Nosema ceranae Spore

ZHOU DingDing,SHI XiaoYu,WANG Jie,FAN YuanChan,ZHU ZhiWei,JIANG HaiBin,FAN XiaoXue,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,XU GuoJun,CHEN DaFu,GUO Rui

表1 本研究使用的引物

Table 1 Primers used in this study

核酸ID Nucleotide ID 引物名称 Primer name 引物序列 Primer sequence (5′-3′) 预期目的片段 Product size (bp) gene1439 1-F ATGTATCTTATGCCAACGC 382 1-R TGAATCTTTCCTACACCCT gene565 2-F TCAAGCCTGTTAAGATGAC 499 2-R ACTACTTCTGGCGGTTTAT gene1719 3-F ATAGGAGGTAAACATCAG 438 3-R ATAGTAAAGTTCAGTGCC gene1366 4-F TTGAAGAAGGTGTCGCAGAAC 182 4-R AGCAGAGCCACATAAACAAGC gene1360 5-F AAGAAGCCGAAACAACCC 101 5-R GTTAGCCGCATCCATAGC gene1134 6-F TGTACGGTCTGGCTGCTTC 352 6-R GGACTTGGCTTTCCACTAA gene1695 7-F GATACAGCCTCTTTGG 138 7-R GGAACAATAACCCTAAA gene1563 8-F TGCCTACAATCAACTCTAAT 384 8-R CACCCAATACTTCAAATAAC MSTRG.3636.1 9-F TAACTAGCCTACTCTATCCC 257 9-R TAAAAGCACTAACTACAACC MSTRG.4883.1 10-F CTGACAGACCATAGTCGGCATA 128 10-R TCTACAATGATGGGCACAGGA nce-miR-8565 11-L CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCTAAAGA 67 11-F CGCGCCTTTAATTGTGAAACATG nce-miR-7502 12-L CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACTTGTC 66 12-F CGCGCCTAAAAACAAAAATGTA nce-miR-7729 13-L CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCTAGGCC 62 13-F CGCGCCTAGTGATGGCTGT - R CTCAACTGGTGTCGTGGA