莲草直胸跳甲Halloween基因家族鉴定及表达分析

刘亦然,张虹,靳继苏,周忠实,郭建英

Identification and Expression Analysis of the Halloween Gene Family in Agasicles hygrophila

LIU YiRan,ZHANG Hong,JIN JiSu,ZHOU ZhongShi,GUO JianYing

表2 用于检测莲草直胸跳甲中Halloween基因表达水平所用引物

Table 2 Primers used to detect the expression level of the Halloween genes in A. hygrophila

基因 Gene 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) AhCYP302A1 GATGAGAGCGATGTAACTGAGG TCGGGTATGAATTTGTCTGGAG AhCYP306A1 CGTAAGCCATCCATAGAAG AGGTCTGATTCGCATAGT AhCYP307A1 ACTTTGGCGTTTTGTGATTGG TGGGTTTCTGTCAAGATAACGG AhCYP307A2 ACTTGCAGAGGACGCG GGAGGGGAATTGTAGTCC AhCYP314A1 GACTTCTTGTTCGTTGGTTC TGTAGATGGTCTCTTCGCTA AhCYP315A1 Actin TCGGTGGTTCTAACTATAAG AGGTATTGCCGACAGAAT AGGACAACAGTATCATCTAAT TGCTGGAAGGTGGATAAG