莲草直胸跳甲Halloween基因家族鉴定及表达分析

刘亦然,张虹,靳继苏,周忠实,郭建英

Identification and Expression Analysis of the Halloween Gene Family in Agasicles hygrophila

LIU YiRan,ZHANG Hong,JIN JiSu,ZHOU ZhongShi,GUO JianYing

表1 用于克隆莲草直胸跳甲中Halloween基因的完整或部分ORF引物

Table 1 Complete or partial ORF primers for the cloning of the Halloween genes in A. hygrophila

基因 Gene 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) AhCYP302A1 CACAGTTTTGGAAAAATACC TTATCTTCTAATAAACTC AhCYP306A1 ATGATGATTACTTATCTAGTGG CTAACCTGCTTGAAAAGGCA AhCYP307A1 GCACATACATTTCCACGAGC TTATCTTTGTGCAAATTT AhCYP307A2 ATGCTCGCGCTCGTTGTG TCAAGATCTGGGAGTTAGC AhCYP314A1 ATGATAGACGAAAGCCTGTAC CTAAACTCTTTCTCTAA AhCYP315A1 ATGTTTTTTCACGTAAAACAATT TTAATCGATTTTACGAAACACC