柑橘溃疡病抗性相关转录因子CitMYB20的功能

姚利晓,范海芳,张庆雯,何永睿,许兰珍,雷天刚,彭爱红,李强,邹修平,陈善春

Function of Citrus Bacterial Canker Resistance-Related Transcription Factor CitMYB20

YAO LiXiao,FAN HaiFang,ZHANG QingWen,HE YongRui,XU LanZhen,LEI TianGang,PENG AiHong,LI Qiang,ZOU XiuPing,CHEN ShanChun

表1 所用引物序列

Table 1 The primer sequences

引物名称 Primer name 用途 Amplification 引物序列 Primer sequence (5′-3′) 备注 Remark CitMYB20-F 克隆基因开放阅读框 To clone ORF sequence ATAGGATCCATGGGGAGGGCTCCCTG BamH I酶切位点 BamH I restriction site CitMYB20-R CCGGAATTCCTAAAATGGTAATGTTAATGAGTCTGC EcoR I酶切位点 EcoR I restriction site CitMYB20-F (g) 克隆基因抑制表达片段 To clone RNAi fragment sequence GCTCTAGAGGCGCGCCAAGCAAAACCAGAAGGCC Xba I和Asc I酶切位点 Xba I and Asc I restriction site CitMYB20-R (g) CGCGGATCCATTTAAATACCGATCGCAAATTCATTAAG BamH I和Sma I酶切位点 BamH I and Sma I restriction site CitMYB20-F (P) 克隆启动子 To clone promoter sequence CCAGAACCTTTACTTTAATTTCTATTTTTA CitMYB20-R (P) CTTGTTAATTTCTTTCAAAGTAGTGGAG CitMYB20-F (q) 检测CitMYB20的表达量 To detect CitMYB20 expression CTCCTCGGTCACTACTGGAGA CitMYB20-R (q) CATTAAGGCCGCCTCCGAAA actin-f 检测actin的表达量 To detect actin expression CATCCCTCAGCACCTTCC actin-r CCAACCTTAGCACTTCTCC 35S-F 鉴定过表达植株 To identify over-expressed plant GGAGTCAAAGATTCAAATAGAGGACCTAAC CitMYB20-R (OE) TGACCACCTATTCCCCAACATGT CitMYB20-F (RNAi) 鉴定RNAi抑制表达植株 To identify RNAi plant ATTTGCGATCGGTATTTAAATGTGTAA CitMYB20-R (RNAi) GCTAGCCAGGATCCAAATACCTGCAAA