利用野生甘蓝改良油菜Ogu CMS恢复材料的菌核病抗性
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万华方,丁一娟,陈致富,梅家琴,钱伟
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Improvement of the Resistance Against Sclerotinia sclerotiorum in Ogu CMS Restorer in Brassica napus Using Wild B. oleracea as Donor
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WAN HuaFang,DING YiJuan,CHEN ZhiFu,MEI JiaQin,QIAN Wei
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表1 分子标记辅助选择的SSR标记
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Table 1 SSR markers used in marker-assisted selection for resistant QTL
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序号
No. | 标记
Marker | 抗病QTL*
Resistant QTL | 连锁群
Linkage group | R2(%) | 引物序列
Primer sequence (5′-3′) | | 1 | Na10-H06 | qLR09-3/qLR10-1 | C1 | 13.6 | F：AGAATGAGACCCAGAAACCG
R：GCCACACTCTCTCTTACTAGGGC | | 2 | SWUC150 | qLR09-3/qLR10-1 | C1 | 13.6 | F：AGATCCTTGCAATTTCGCAC
R：TCCTGCAAAGCTTCATCCTC | | 3 | SWUC455 | qLR09-4/qLR10-2 | C3 | 10.8 | F：GACAACACAACAGACGCA
R：GCATTTCCCATTACTTCCA | | 4 | SWUC427 | qSR10-2 | C7 | 3.4 | F：CCTCTGTTTCTTTGCTCTTTG
R：GATTCATTGTGTGTGTGATGT | | 5 | SWUC260 | qSR09-1/qSR10-3 | C9 | 16.1 | F：AACGGCGCTCTAGAGGAAAT
R：AGCTGAACAGCTTGATTGGG | | 6 | SWUC663 | qSR09-1/qSR10-3 | C9 | 16.1 | F：TGCAGTCGGAATAACCATCA
R：TTCCTATTCTCCAACCGCAG | | 7 | SWUC934 | qLR09-2 | C9 | 24.1 | F：TTCACACTCCCGATCTCTCC
R：AGATTCCCTCCCCAACAAGT |
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